l casei atcc 27139 (ATCC)
ATCC is a verified supplier 
ATCC manufactures this product 
96
Structured Review
ATCC
l casei atcc 27139
L Casei Atcc 27139, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 125 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/l+casei+atcc+27139/Lacticaseibacillus+paracasei+(Collins+et+al%2E)+Zheng+et+al/pmc03885529-141-1-3
Average 96 stars, based on 125 article reviews
L Casei Atcc 27139, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 125 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/l+casei+atcc+27139/Lacticaseibacillus+paracasei+(Collins+et+al%2E)+Zheng+et+al/pmc03885529-141-1-3
Average 96 stars, based on 125 article reviews
l casei atcc 27139 - by Bioz Stars,
2026-09
96/100 stars
Images
1) Product Images from "Transposon Mutagenesis of Probiotic Lactobacillus casei Identifies asnH , an Asparagine Synthetase Gene Involved in Its Immune-Activating Capacity"
Article Title: Transposon Mutagenesis of Probiotic Lactobacillus casei Identifies asnH , an Asparagine Synthetase Gene Involved in Its Immune-Activating Capacity
Journal: PLoS ONE
doi: 10.1371/journal.pone.0083876
Figure Legend Snippet: Summary of relative sensitivities to J1 phage infection and predicted transposon insertion loci of J1 phage-resistant mutant.
Techniques Used: Infection, Mutagenesis
Figure Legend Snippet: Heat-killed preparations of L. casei ATCC 27139 or isogenic Tn 5 insertion mutants (800 µg) were injected i.v. into BALB/c mice 6 days before an i.v. challenge with L. monocytogenes (2.0×10 6 CFU/mouse). Six mice per group were dissected 24 h after the challenge, and viable Listeria were detected in spleens. Columns: white, saline; black, L. casei wild-type; slashed, L. casei J1 phage-resistant mutant; gray, L. casei asnH mutant complemented with the cloned asnH . Results are depicted as the means ± standard deviations (SD). Statistical significance was calculated using the Student's t -test. Significant differences indicated between the control and treated groups. ***, p <0.001.
Techniques Used: Injection, Mutagenesis, Clone Assay
Figure Legend Snippet: Heat-killed preparations of L. casei ATCC 27139 or isogenic Tn 5 insertion mutants (800 µg) were injected i.v. into BALB/c mice. Six mice per group were sacrificed 8 h after injection, and IL-12 (A), TNF-α (B), and IFN-γ (C) proteins were measured from spleen homogenates by ELISA. Columns: white, saline; black, L. casei wild-type; slashed, L. casei J1 phage-resistant mutant; gray, L. casei asnH mutant complemented with the cloned asnH . Results are depicted as the means ± standard deviations (SD). Statistical significance was calculated using the Student's t -test. Significant differences indicated between the control and treated groups. ***, p <0.001.
Techniques Used: Injection, Enzyme-linked Immunosorbent Assay, Mutagenesis, Clone Assay
Figure Legend Snippet: (A) Protein sequence alignment was performed using the GENETYX program. Solid boxes indicate highly conserved regions. Regions containing residues important for enzymatic activities are shown in detail (identical residues are indicated by boldface type). The first methionine was consistently included in the numbering and was designated Met-1. Asterisks indicate mutated residues in this study that were critical to glutaminase or synthetase activities. (B) Recombinant AsnH WT , mutant AsnH C2S , and mutant AsnH D265N (0.1 µg) were examined using the glutaminase activity test. Five microliters of a reaction mixture (initial volume, 50 µl) were quenched at 30 min to estimate glutamic acid concentration. Glutaminase activity was monitored in the presence of aspartic acid or cell wall extracts of ATCC 27139, ATP or AMP–PNP plus cell wall extracts. Results are depicted as the means ± standard deviations (SD). Statistical significance was calculated using the Student's t -test. Significant differences indicated between the control and treated groups. ***, p <0.001.
Techniques Used: Sequencing, Recombinant, Mutagenesis, Activity Assay, Concentration Assay
Figure Legend Snippet: Amino acid compositions in peptidoglycans and lysozyme MICs of J1 phage-resistant mutants.
Techniques Used:
Figure Legend Snippet: Relative sensitivities to J1 phage infection among MNNG-induced J1 phage-resistant mutants.
Techniques Used: Infection
Related Articles
Adsorption:Article Title: Exposing the Secrets of Two Well-Known Lactobacillus casei Phages, J-1 and PL-1, by Genomic and Structural Analysis Article Snippet: .. J-1 gp16 exhibited a higher affinity than PL-1 gp16 for cell walls of Inhibition:Article Title: Exposing the Secrets of Two Well-Known Lactobacillus casei Phages, J-1 and PL-1, by Genomic and Structural Analysis Article Snippet: .. J-1 gp16 exhibited a higher affinity than PL-1 gp16 for cell walls of Activity Assay:Article Title: Transposon Mutagenesis of Probiotic Lactobacillus casei Identifies asnH , an Asparagine Synthetase Gene Involved in Its Immune-Activating Capacity Article Snippet: .. In AsnH of Article Title: Bio-functional properties of probiotic Lactobacillus : current applications and research perspectives. Article Snippet: Lactic acid bacteria as a starter culture are very important component in the fermentation process of dairy and food industry.. Application of lactic acid bacteria as probiotic bacteria adds more functionality to the developed product.. Gut colonizing bacteria have attractive benefits related to human health. Article Title: Transposon Mutagenesis of Probiotic Lactobacillus casei Identifies asnH , an Asparagine Synthetase Gene Involved in Its Immune-Activating Capacity Article Snippet: .. Peptidoglycan and derived muropeptides in L. salivarius showed protective effect in murine colitis mice model through induction of pathogen-associated molecular pattern receptors such as Nod2 , which recognizes intracellular muramyl dipeptide of a peptidoglycan constituent , suggesting the relevance of peptidoglycan structure to the immune-augmenting activity of Mouse Assay:Article Title: Transposon Mutagenesis of Probiotic Lactobacillus casei Identifies asnH , an Asparagine Synthetase Gene Involved in Its Immune-Activating Capacity Article Snippet: .. Mutagenesis:Article Title: Translating Omics to Food Microbiology. Article Snippet: This review examines the applications of omics technologies in food microbiology, with a primary focus on high-throughput sequencing (HTS) technologies.. We discuss the different sequencing approaches applicable to the study of food-related microbial isolates and mixed microbial communities in foods, and we provide an overview of the sequencing platforms suitable for each approach.. We highlight the potential for genomics, metagenomics, and metatranscriptomics to guide efforts to optimize food fermentations. Article Title: Transposon Mutagenesis of Probiotic Lactobacillus casei Identifies asnH , an Asparagine Synthetase Gene Involved in Its Immune-Activating Capacity Article Snippet: Recently, we developed a random mutagenesis system using a Tn 5 -based EZ::TN transposome for the probiotic L. casei ATCC 27139 . .. Using this system, we constructed a Tn 5 insertion library of nearly 10,000 transposon insertion mutants of Infection:Article Title: Translating Omics to Food Microbiology. Article Snippet: This review examines the applications of omics technologies in food microbiology, with a primary focus on high-throughput sequencing (HTS) technologies.. We discuss the different sequencing approaches applicable to the study of food-related microbial isolates and mixed microbial communities in foods, and we provide an overview of the sequencing platforms suitable for each approach.. We highlight the potential for genomics, metagenomics, and metatranscriptomics to guide efforts to optimize food fermentations. Derivative Assay:Article Title: Transposon Mutagenesis of Probiotic Lactobacillus casei Identifies asnH , an Asparagine Synthetase Gene Involved in Its Immune-Activating Capacity Article Snippet: .. Peptidoglycan and derived muropeptides in L. salivarius showed protective effect in murine colitis mice model through induction of pathogen-associated molecular pattern receptors such as Nod2 , which recognizes intracellular muramyl dipeptide of a peptidoglycan constituent , suggesting the relevance of peptidoglycan structure to the immune-augmenting activity of Construct:Article Title: Transposon Mutagenesis of Probiotic Lactobacillus casei Identifies asnH , an Asparagine Synthetase Gene Involved in Its Immune-Activating Capacity Article Snippet: Recently, we developed a random mutagenesis system using a Tn 5 -based EZ::TN transposome for the probiotic L. casei ATCC 27139 . .. Using this system, we constructed a Tn 5 insertion library of nearly 10,000 transposon insertion mutants of Genome Wide:Article Title: Transposon Mutagenesis of Probiotic Lactobacillus casei Identifies asnH , an Asparagine Synthetase Gene Involved in Its Immune-Activating Capacity Article Snippet: Recently, we developed a random mutagenesis system using a Tn 5 -based EZ::TN transposome for the probiotic L. casei ATCC 27139 . .. Using this system, we constructed a Tn 5 insertion library of nearly 10,000 transposon insertion mutants of |